Mary Biorxiv
Harumi Biorxiv
Delivery Review
Nelson CE, We Y, Gemberling MP, Oliver ML, Waller MA, Bohning JD, Robinson-Hamm JN, Bulaklak K, Castellanos Rivera RM, Collier JH, Asokan A, Gersbach CA. Long-term Evaluation of AAV-CRISPR Genome Editing for Duchenne Muscular Dystrophy . 25, pages 427–432 (2019) Article
Nelson CE, Hakim C, Ousterout DG, Thakore PI, Moreb EA, Castellanos R, Madhavan S, Xiufang P, Ran A, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy. Science. 2016, 351(6271): 403-407
– Highlighted by New York Times (Jan 1, 2016, A13) | Nature 529:130 2016 | Science 351:6271 2016 Nature Rev Genet 17:124 2016 | Nature Rev Neurol 12:63 2016 | Nature Rev Drug Discov 15:160 2016 | Mol Ther 24:3 2016 | N Engl J Med 374 2016 | NIH Research Matters | The Guardian | BBC | Stat News | GEN News | Rare Disease Report | F1000
Nelson CE, Kim AJ, Adolph EJ, Gupta MK, Yu F, Hocking KM, Davidson JM, Guelcher SA, Duvall CL. Tunable Delivery of siRNA from a Biodegradable Scaffold to Promote Angiogenesis in Vivo. Advanced Materials. 2014; 26(4): 607-614 – Featured on Inside Front Cover
– Highlighted by NIBIB and Fierce Drug Delivery (2014-01-22)
Nelson CE*, Kintzing JR*, Hanna A, Shannon JM, Gupta MK, Duvall CL. Balancing Cationic and Hydrophobic Content of PEGylated siRNA Polyplexes Enhances Endosome Escape, Stability, Blood Circulation Time and Bioactivity In Vivo. ACS Nano. 2013, 7(10): 8870-8880.
Nelson CE, Gupta MK, Adolph EJ, Shannon JM, Guelcher SA, Duvall CL. Sustained local delivery of siRNA from an injectable scaffold. Biomaterials. 2012; 33(4): 1154-61
– Research Highlight: Timko BP, Kohane DS, “Injectable Scaffolds Achieve Sustained siRNA Release for Tissue Repair.” Nanomedicine. 2012; 7(3): 315-316
Primary Research
Hakim CH*, Wasala NB*, Nelson CE, Wasala LP, Yue Y, Louderman JA, Lessa TB, Dai A, Zhang K, Jenkins GJ, Nance ME, Pan X, Kodippili K, Yang NN, Chen S, Gersbach CA, Duan D. AAV CRISPR editing rescues cardiac and muscle function for 18 months in dystrophic mice. JCI Insight. 3(23):e124297 (2018). Article
Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA. RNA-Guided Transcriptional Silencing In Vivo with S. aureus CRISPR-Cas9 Repressors. Nature Communications. 9:1674 (2018). Article
Huang J, Chen M, Whitley MJ, Kuo HC, Xu, ES, Walens A, Mowery YM, Van Mater D, Eward WC, Cardona DM, Luo L, Ma Y, Lopez OM, Nelson CE, Robinson-Hamm JN, Reddy A, Dave SS, Gersbach CA, Dodd RD, and Kirsch DG. Generation and Comparison of CRISPR/Cas9 and Cre-Mediated Genetically Engineered Mouse Models of Sarcoma. Nature Communications. 8, 15999 (2017). Article
Martin JR, Nelson CE, Gupta MK, Yu F, Hocking KM, Davidson JM, Guelcher SA, Duvall CL. Local delivery of PHD2 siRNA from ROS-degradable scaffolds to promote diabetic wound healing. Advanced Healthcare Materials. 5(21):2751-2757 (2016). Article
Werfel TA, Swain C, Nelson CE, Kilchrist KV, Miteva M, Duvall CL. Hydrolytic charge-reversal of PEGylated polyplexes enhances intracellular un-packaging and activity of siRNA. Journal of Biomedical Materials Research: Part A. 2016; 104(4): 917-27.
Gupta MK, Lee SH, Crowder SW, Wang X, Hofmeister L, Nelson CE, Bellan L, Duvall CL, Sung HJ. Oligoproline-Derived Nanocarrier for Dual Stimuli-Responsive Gene Delivery. Journal of Materials Chemistry B. 2015; 3: 7271-7280
Li HM, Miteva M, Kirkbride KC, Cheng MJ, Nelson CE, Simpson EM, Gupta MK, Duvall CL, Giorgio TD. Dual MMP7-Proximity-Activated and Folate Receptor targeted Nanoparticles for siRNA Delivery. Biomacromolecules. 2015; 16(1): 192–201.
Poole KM, Nelson CE, Joshi RV, Martin JR, Gupta MK, Haws SC, Kavanaugh TE, Skala MC, Duvall CL. ROS-responsive Microspheres for on Demand Antioxidant Therapy in a Model of Diabetic Peripheral Arterial Disease. Biomaterials. 2015; 41(0): 166-175.
Poole KM, Patil CA, Nelson CE, McCormack DR, Madonna MC, Duvall CL, Skala MC. Longitudinal Study of Arteriogenesis with Swept Source Optical Coherence Tomography and Hyperspectral Imaging. Proc SPIE. 2014; 8934. doi: 10.1117/12.2042037
Miteva M, Kirkbride KC, Kilchrist KV, Werfel TA, Li H, Nelson CE, Gupta MK, Giorgio TD, Duvall CL. Tuning Pegylation of Mixed Micelles to Overcome Intracellular and Systemic sirna Delivery Barriers. Biomaterials. 2015; 38: 97–107.
Adolph EJ, Nelson CE, Werfel TA, Guo R, Davidson J, Guelcher SA, Duvall CL. Enhanced Performance of Plasmid DNA Polyplexes Stabilized by a Combination of Core Hydrophobicity and Surface PEGylation. Journal of Materials Chemistry B. 2014; 2:8154-8164.
Joshi RV*, Nelson CE*, Poole KM, Skala MC, Duvall CL. Dual pH- and Temperature-Responsive Microparticles for Protein Delivery to Ischemic Tissues. Acta Biomaterialia. 2013; 9(5):6526-34 *Equally Contributing 1st authors.
Yu SS, Lau C, Barham W, Onishko H, Nelson CE, Li HM, Yull F, Duvall CL, Giorgio TD. Macrophage-Specific RNAi Targeting via ‘Click’, Mannosylated Polymeric Micelles. Molecular Pharmaceutics. 2013; 10(3): 975-87
Li HM, Yu SS, Miteva M, Nelson CE, Werfel T, Giorgio TD, Duvall CL. Matrix Metalloproteinase Responsive, Proximity-activated Targeting Polymeric Nanoparticles for siRNA Drug Carrier. Advanced Functional Materials. 2013; 23(24): 3040-3052.
Gupta MK, Meyer TA, Nelson CE, Duvall CL. Poly (PS-b-DMA) Micelles for Reactive Oxygen Species Triggered Drug Release. Journal of Controlled Release. 2012; 162(3):591-8
Nelson CE, Moon HM, Kim JW. Carbon Nanotube Cluster Based Micro-Fluidic System for Bacteria Capture, Concentration, and Separation. Inquiry: Undergraduate Research Journal of the University of Arkansas, 2009; 10.
Reviews
Nelson CE, Robinson-Hamm JN, Gersbach CA. Genome Engineering: A new Approach for Gene Therapy in Neuromuscular Disorders. Invited review for Nature Reviews Neurology. 2017; 13, 647–661.
Nelson CE, Gersbach CA. Cas9 loosens its grip on off-target sites: two new variants of the Cas9 nuclease have minimal activity at off-target DNA sites. Nature biotechnology. 2016; 34(3), 298-300.
Nelson CE, Gersbach CA. Engineering Delivery Vehicles for Genome Editing. Annual Review of Chemical and Biomolecular Engineering. 2016; Vol. 7:637-62.
Sarrett SM, Nelson CE, Duvall CL. Technologies for Controlled, Local Delivery of siRNA. Journal of Controlled Release. 2015; 28;218:94-113
Beavers KR, Nelson CE, Duvall CL. MiRNA Inhibition in Tissue Engineering and Regenerative Medicine. Advanced Drug Delivery Reviews. 2015; 88(1): 123-137.
Sarett SM, Beavers KR, Miteva M, Nelson CE, Duvall CL. Research Highlights: Highlights from the Latest Articles in Nanomedicine. Nanomedicine. 2013; 8(3): 329-32
Evans BC*, Nelson CE*, Yu SS*, Kim AJ, Li HM, Nelson HM, Giorgio TD, Duvall CL. Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs. JoVE. 2013; 73:e50166 *Equally Contributing 1st authors.
Nelson CE, Gupta MK, Adolph EJ, Guelcher SA, Duvall CL. siRNA Delivery from an Injectable Scaffold for Wound Therapy. Advances in Wound Care. 2013; 2(3):93-99
Li HM*, Nelson CE*, Evans BC*, Duvall CL. Delivery of Intracellular-acting Biologics in Pro-Apoptotic Therapies. For a special issue entitled “Development of drugs interfering with apoptosis.” Current Pharmaceutical Design. 2011; 17(3):293-319.
Book Chapters
1. Nelson CE, Gersbach CA. Genetic Repair therapy for DMD. Muscle Gene Therapy. In press